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MSE Seminar Series: Paul Paukstelis 3D DNA Crystals: Assembly and Application DNA has proved to be a successful material for creation of nanoscale structures because of its inherent programmability and predictable structural features. Though it has been used extensively to create discrete objects in two- and three-dimensions, the self-assembly of DNA into macroscopic periodic structures has proved much more difficult. Our laboratory is exploring how predictable non-Watson-Crick base pairs can be utilized as junctional motifs to generate crystals with 3D connectivity and useful nanoscale features. We have described several 3D DNA crystal structures -- mediated by non-canonical base pairs -- that contain solvent channels that run through the length of the crystal in multiple direction. These porous crystals allow incorporation of proteins, and we have recently demonstrated that an enzyme encapsulated in these channels retains its catalytic activity. We have also described a pH-responsive non-canonical junction motif that can be activated after crystal formation to initiate concerted changes in the crystal unit cell dimensions. We continue to discover new non-canonical DNA motifs in hopes of developing a structural library that will allow us to custom design crystals for uses as macromolecular scaffolds or environmentally responsive biomaterials. About the Speaker This Event is For: Campus |