| Description |
Features and Specifications:
- Custom-built microanalytical capillary electrophoresis platform capable of analyzing 1-50 nL portion from <1 μL samples
- Commercial nano-flow liquid chromatograph (Dionex Ultimate 3000)
- Custom-built CE nano-flow electrospray ionization source capable of efficiently ionizing molecules separated by CE
- A quadrupole-linear ion trap-orbitrap tribrid analyzer ultrahigh-resolution mass spectrometer (Thermo Orbitrap Lumos) equipped with a collision-induced dissociation (CID), a higher-energy collisional dissociation (HDC), and an electron transfer dissociation (ETD) cell, enabling 240,000-500,000 HFWM spectral resolution
- Capability for tandem and multistage mass spectrometry (MS2, MSn)
- Comprehensive suite of metabolomics and proteomics software packages to process mass spectrometry datasets
Potential Applications:
- Virtually any compound that can be ionized for mass spectrometry, including most biomolecules (e.g., proteins, peptides, and metabolites) and manmade compounds (e.g., organic compounds, polymers)
- Particularly recommended for proteins and peptides
- Qualitative analysis to determine what types of molecules are present in the sample
- Quantitative analysis to determine how much of the molecules are present in the sample
- Multiplexing quantification for proteomics/peptidomics using tandem mass tags (TMT, Thermo Scientific) or Isobaric tag for relative and absolute quantitation (iTRAQ) reagents
- Representative results using this or a similar platform:
- A. B. Baxi, C. Lombard-Banek, S. A. Moody, and P. Nemes, Proteomic characterization of the neural ectoderm fated cell clones in the Xenopus laevis embryo by high-resolution mass spectrometry, ACS Chem. Neurosci. 2018, 9, 2064-2073, DOI: 10.1021/acschemneuro.7b00525
- S. B. Choi, C. Lombard-Banek, P. Munoz-LLancao, M. C. Manzini, and P. Nemes, Enhanced peptide detection toward single-neuron proteomics by reversed-phase fractionation capillary electrophoresis mass spectrometry, J. Am. Soc. Mass Spectrom. 2017, 29, 913-922, DOI: 10.1007/s13361-017-1838-1
- S. B. Choi, C. Lombard-Banek, P. Munoz-LLancao, M. C. Manzini, and P. Nemes, Enhanced peptide detection toward single-neuron proteomics by reversed-phase fractionation capillary electrophoresis mass spectrometry, J. Am. Soc. Mass Spectrom. 2017, 29, 913-922, DOI: 10.1007/s13361-017-1838-1
- C. Lombard-Banek, Sally A. Moody, and P. Nemes, Label-free quantification of proteins in single embryonic cells with neural fate in the cleavage-stage frog (Xenopus laevis) embryo using capillary electrophoresis electrospray ionization high resolution mass spectrometry (CE-ESI-HRMS), Mol. Cell. Prot. 2016, 15, 2756-2768, DOI: 10.1074/mcp.M115.057760
- C. Lombard-Banek, S. A. Moody, and P. Nemes, Single-cell mass spectrometry for discovery proteomics: quantifying translational cell heterogeneity in the 16-cell frog (Xenopus) embryo, Angew. Chem. Int. Ed. 2016, 55, 2454-2458, DOI: 10.1002/anie.201510411, PMID 26756663
Rates:
UMD Rate: $100/h for instrument time, assistance, and data analysis
External Non-Profit/University: $155/h for instrument time, assistance, and data analysis
Commercial: $200/h for instrument time, assistance, and data analysis
Instrument time is charged per hour; assistance and data analysis are charged in half-hour increments
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Features and Specifications:
