November 7, 2024 UMD Home FabLab AIMLab
Microanalytical Ultrahigh-Resolution Orbitrap Mass Spectrometer System Back to Equipment List
Operational Status ● Online
Description Features and Specifications:
  • Custom-built microanalytical capillary electrophoresis platform capable of analyzing 1-50 nL portion from <1 μL samples
  • Commercial nano-flow liquid chromatograph (Dionex Ultimate 3000)
  • Custom-built CE nano-flow electrospray ionization source capable of efficiently ionizing molecules separated by CE
  • A quadrupole-linear ion trap-orbitrap tribrid analyzer ultrahigh-resolution mass spectrometer (Thermo Orbitrap Lumos) equipped with a collision-induced dissociation (CID), a higher-energy collisional dissociation (HDC), and an electron transfer dissociation (ETD) cell, enabling 240,000-500,000 HFWM spectral resolution
  • Capability for tandem and multistage mass spectrometry (MS2, MSn)
  • Comprehensive suite of metabolomics and proteomics software packages to process mass spectrometry datasets
Potential Applications:
  • Virtually any compound that can be ionized for mass spectrometry, including most biomolecules (e.g., proteins, peptides, and metabolites) and manmade compounds (e.g., organic compounds, polymers)
  • Particularly recommended for proteins and peptides
  • Qualitative analysis to determine what types of molecules are present in the sample
  • Quantitative analysis to determine how much of the molecules are present in the sample
  • Multiplexing quantification for proteomics/peptidomics using tandem mass tags (TMT, Thermo Scientific) or Isobaric tag for relative and absolute quantitation (iTRAQ) reagents
  • Representative results using this or a similar platform:
    • A. B. Baxi, C. Lombard-Banek, S. A. Moody, and P. Nemes, Proteomic characterization of the neural ectoderm fated cell clones in the Xenopus laevis embryo by high-resolution mass spectrometry, ACS Chem. Neurosci. 2018, 9, 2064-2073, DOI: 10.1021/acschemneuro.7b00525
    • S. B. Choi, C. Lombard-Banek, P. Munoz-LLancao, M. C. Manzini, and P. Nemes, Enhanced peptide detection toward single-neuron proteomics by reversed-phase fractionation capillary electrophoresis mass spectrometry, J. Am. Soc. Mass Spectrom. 2017, 29, 913-922, DOI: 10.1007/s13361-017-1838-1
    • S. B. Choi, C. Lombard-Banek, P. Munoz-LLancao, M. C. Manzini, and P. Nemes, Enhanced peptide detection toward single-neuron proteomics by reversed-phase fractionation capillary electrophoresis mass spectrometry, J. Am. Soc. Mass Spectrom. 2017, 29, 913-922, DOI: 10.1007/s13361-017-1838-1
    • C. Lombard-Banek, Sally A. Moody, and P. Nemes, Label-free quantification of proteins in single embryonic cells with neural fate in the cleavage-stage frog (Xenopus laevis) embryo using capillary electrophoresis electrospray ionization high resolution mass spectrometry (CE-ESI-HRMS), Mol. Cell. Prot. 2016, 15, 2756-2768, DOI: 10.1074/mcp.M115.057760
    • C. Lombard-Banek, S. A. Moody, and P. Nemes, Single-cell mass spectrometry for discovery proteomics: quantifying translational cell heterogeneity in the 16-cell frog (Xenopus) embryo, Angew. Chem. Int. Ed. 2016, 55, 2454-2458, DOI: 10.1002/anie.201510411, PMID 26756663
Rates:
UMD Rate: $100/h for instrument time, assistance, and data analysis
External Non-Profit/University: $155/h for instrument time, assistance, and data analysis
Commercial: $200/h for instrument time, assistance, and data analysis
Instrument time is charged per hour; assistance and data analysis are charged in half-hour increments
Location NanoMass | CHEM 1517/1519
Manufacturer
Rates
UMD
$60/hr
External Non-profit / University
$100/hr
Small Commercial / MTECH
$150/hr
Large Commercial
$150/hr
No Charge
$0/hr
Reservations No upcoming reservations at this time.
Logs
Thu, Dec 07, 2023
9:00 am - 9:30 am
Ryan O'Neal
View Reservation
TI charge $2000 to BBI Project
Wed, Nov 01, 2023
12:00 pm - 12:30 pm
Ryan O'Neal
View Reservation
TI 40 samples $50/sample Total charge $2000 Charge to BBI
Fri, Oct 13, 2023
11:00 am - 12:00 pm
Ryan O'Neal
View Reservation
TI 40 samples $50/sample Total cost: $2000 Charge to BBI
Thu, Aug 31, 2023
10:00 am - 11:00 am
Ryan O'Neal
View Reservation

Deleted by: Ryan O'Neal

BS Total charge: $1000 Charge to BBI
Wed, Aug 30, 2023
10:00 am - 11:00 am
Ryan O'Neal
View Reservation
TI $50/sample 48 samples Total charge: $2400 Charge to BBI
Records to show:
SOPs
Manuals

You must have lab permissions to view the manuals.

Please login to view manuals or contact the lab staff to obtain permissions.

Recipes

Colleges A. James Clark School of Engineering
The College of Computer, Mathematical, and Natural Sciences

Communicate Join Email List
Contact Us
Follow us on TwitterTwitter logo

Links Privacy Policy
Sitemap
RSS

Copyright The University of Maryland University of Maryland
2004-2024