| Description |
Features and Specifications:
- Custom-built microanalytical capillary electrophoresis platform capable of analyzing 1-50 nL portion from <1 μL samples
- Commercial micro-flow liquid chromatograph (Waters Acquity UPLC-I Class)
- Custom-built CE micro-flow electrospray ionization source capable of efficiently ionizing molecules separated by CE
- A quadrupole-time of flight high-resolution mass spectrometer (Impact HD, Bruker Daltonics) equipped with a collision-induced dissociation (CID) cell, enabling up to 40,000 FWHM spectral resolution
- Capability for tandem mass spectrometry (MS2)
- Comprehensive suite of metabolomics and proteomics software packages to process mass spectrometry datasets
Potential Applications:
- Well-suited for metabolites, man-made compounds
- Qualitative analysis to determine what types of molecules are present in the sample
- Quantitative analysis to determine how much of the molecules are present in the sample
- Multiplexing quantification for proteomics/peptidomics using tandem mass tags (TMT, Thermo Scientific) or Isobaric tag for relative and absolute quantitation (iTRAQ) reagents
- Representative results using this or a similar platform:
- E. P. Portero and P. Nemes*, Dual cationic-anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS, Analyst 2019, in print, DOI: 10.1039/C8AN01999A
- R. M. Onjiko, E. P. Portero, S. A. Moody, and P. Nemes*, Microprobe capillary electrophoresis mass spectrometry for single-cell metabolomics in live frog (Xenopus laevis) embryos, J. Vis. Exp. 2017, e56956, DOI: 10.3791/56956
- R. M. Onjiko, D. O. Plotnick, S. A. Moody, and P. Nemes*, Metabolic comparison of dorsal versus ventral cells directly in the live 8-cell frog embryo by microprobe single-cell CE-ESI-MS, 2017, Anal. Methods, 2017, 9, 4964-4970, DOI: 10.1039/C7AY00834A
- R. M. Onjiko, E. P. Portero, S. A. Moody, and P. Nemes*, In situ microprobe single-cell capillary electrophoresis mass spectrometry: Metabolic reorganization in single differentiating cells in the live vertebrate (X. laevis) embryo, Anal. Chem. 2017, 89, 7069-7076, DOI: 10.1021/acs.analchem.7b00880
- R. M. Onjiko, S. Morris, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry with multi-solvent extraction identifies metabolic differences between left and right blastomeres in the 8-cell frog (Xenopus) embryo, Analyst 2016, 141, 3648-3656, DOI: 10.1039/C6AN00200E
- R. M. Onjiko, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo, Proc. Nat. Acad. Sci. USA 2015, 112, 6545-6550, DOI: 10.1073/pnas.1423682112, PMID: 25941375
Rates:
UMD Rate: $100/h for instrument time, assistance, and data analysis
External Non-Profit/University: $125/h for instrument time, assistance, and data analysis
Commercial: $200/h for instrument time, assistance, and data analysis
Instrument time is charged per hour; assistance and data analysis are charged in half-hour increments
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Features and Specifications:
